Composite
Part:BBa_K1916120:Design
Designed by: Jonathan Chen Group: iGEM16_UC_Davis (2016-10-13)
Ara-Inducible CBCR9 Expression System
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 131
Illegal XbaI site found at 212
Illegal SpeI site found at 689 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal SpeI site found at 689 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 131
Illegal XbaI site found at 212
Illegal SpeI site found at 689 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 131
Illegal XbaI site found at 212
Illegal SpeI site found at 689
Illegal NgoMIV site found at 1185
Illegal AgeI site found at 1275 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.
Source
Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.